Volume 25, No 3, Mar 2015

ISSN: 1001-0602 
EISSN: 1748-7838 2018 
impact factor 17.848* 
(Clarivate Analytics, 2019)

Volume 25 Issue 3, March 2015: 386-389

LETTERS TO THE EDITOR

Base-resolution maps of 5-formylcytosine and 5-carboxylcytosine reveal genome-wide DNA demethylation dynamics

Xingyu Lu^1,*, Dali Han^1,*, Zhao Boxuan Simen^1,*, Chun-Xiao Song¹, Li-Sheng Zhang¹, Louis C Doré¹ and Chuan He¹

¹Department of Chemistry and Institute for Biophysical Dynamics, Howard Hughes Medical Institute, The University of Chicago, 929 E 57th Street, Chicago, IL 60637, USA Correspondence: Chuan He,(chuanhe@uchicago.edu)

The TET family of dioxygenases can oxidize 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC), and 5-carboxylcytosine (5caC) in mammalian genomic DNA via a stepwise manner1,2,3,4,5. 5fC and 5caC are selectively recognized and excised by mammalian thymine DNA glycosylase (TDG), and restored to normal cytosine through base excision repair3,6,7,8,9. Once converted to 5fC and 5caC, the modified cytosine base is presumably committed to demethylation through the TDG-dependent pathway or other potential mechanisms. Thus 5fC and 5caC specifically mark active demethylation in the mammalian genome.

10.1038/cr.2015.5

FULL TEXT | PDF

Browse 2194