Volume 25, No 8, Aug 2015
ISSN: 1001-0602
EISSN: 1748-7838 2018
impact factor 17.848*
(Clarivate Analytics, 2019)
Volume 25 Issue 8, August 2015: 946-962 | Open Access
ORIGINAL ARTICLES
Mutation of kri1l causes definitive hematopoiesis failure via PERK-dependent excessive autophagy induction
Xiao-E Jia1,4,*, Ke Ma1,*, Tao Xu1,*, Lei Gao1, Shuang Wu1, Cong Fu1, Wenjuan Zhang1, Zhizhang Wang1, Kaiyu Liu6, Mei Dong1, Changbin Jing1, Chunguang Ren1, Zhiwei Dong1, Yi Chen2, Yi Jin2, Qiuhua Huang2, Xing Chang1,9, Min Deng1, Li Li8, Lingfei Luo8, Jun Zhu7,9, Yongjun Dang6, Hung-Chun Chang5, Leonard I Zon3, Yi Zhou3, Saijuan Chen2,9 and Weijun Pan1,9
1Key Laboratory of Stem Cell Biology, Institute of Health Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences & Shanghai Jiao Tong University School of Medicine, Shanghai, China
2State Key Laboratory for Medical Genomics, Shanghai Institute of Hematology, RuiJin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
3Stem Cell Program, Hematology/Oncology Program at Children's Hospital Boston, Harvard Medical School, Boston, MA 02114, USA
4Biomedical Research Center, Baotou Medical College, Baotou, Inner Mongolia Autonomous Region, China
5Institute of Neuroscience, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China
6Key Laboratory of Metabolism and Molecular Medicine, Ministry of Education, Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Fudan University, Shanghai, China
7CNRS LIA, Shanghai Institute of Hematology, RuiJin Hospital, 197 RuiJin Road II, Shanghai, China
8Key Laboratory of Freshwater Fish Reproduction and Development, Ministry of Education, Laboratory of Molecular Developmental Biology, School of Life Sciences, Southwest University, Beibei, Chongqing, China
9Collaborative Innovation Center of Systems Biomedicine, Shanghai Jiao Tong University School of Medicine, Shanghai, China
Correspondence: Weijun Pan, E-mail: weijunpan@sibs.ac.cn; Saijuan Chen, E-mail: sjchen@stn.sh.cn; Yi Zhou,(yzhou@enders.tch.harvard.edu)
Dysregulation of ribosome biogenesis causes human diseases, such as Diamond-Blackfan anemia, del (5q-) syndrome and bone marrow failure. However, the mechanisms of blood disorders in these diseases remain elusive. Through genetic mapping, molecular cloning and mechanism characterization of the zebrafish mutant cas002, we reveal a novel connection between ribosomal dysfunction and excessive autophagy in the regulation of hematopoietic stem/progenitor cells (HSPCs). cas002 carries a recessive lethal mutation in kri1l gene that encodes an essential component of rRNA small subunit processome. We show that Kri1l is required for normal ribosome biogenesis, expansion of definitive HSPCs and subsequent lineage differentiation. Through live imaging and biochemical studies, we find that loss of Kri1l causes the accumulation of misfolded proteins and excessive PERK activation-dependent autophagy in HSPCs. Blocking autophagy but not inhibiting apoptosis by Bcl2 overexpression can fully rescue hematopoietic defects, but not the lethality of kri1lcas002 embryos. Treatment with autophagy inhibitors (3-MA and Baf A1) or PERK inhibitor (GSK2656157), or knockdown of beclin1 or perk can markedly restore HSPC proliferation and definitive hematopoietic cell differentiation. These results may provide leads for effective therapeutics that benefit patients with anemia or bone marrow failure caused by ribosome disorders.
10.1038/cr.2015.81
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