Volume 26, No 1, Jan 2016

ISSN: 1001-0602 
EISSN: 1748-7838 2018 
impact factor 17.848* 
(Clarivate Analytics, 2019)

Volume 26 Issue 1, January 2016: 131-134

LETTERS TO THE EDITOR

Parthenogenetic haploid embryonic stem cells efficiently support mouse generation by oocyte injection

Cuiqing Zhong^1,2,3,*, Zhenfei Xie^1,2,3,*, Qi Yin^1,2,3,*, Rui Dong^3,4, Suming Yang^1,2,3, Yuxuan Wu^1,2, Li Yang⁴ and Jinsong Li^1,2

¹Group of Epigenetic Reprogramming, State Key Laboratory of Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China
²Shanghai Key Laboratory of Molecular Andrology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China
³University of Chinese Academy of Sciences, Beijing 100049, China
⁴Key Laboratory of Computational Biology, CAS-MPG Partner Institute for Computational Biology; CAS Center for Excellence in Brain Science, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China Correspondence: jsli@sibcb.ac.cn

Mammalian haploid embryonic stem cells (haESCs) have been recently generated from parthenogenetic and androgenetic embryos1,2. Both parthenogenetic haESCs (PG-haESCs) and androgenetic haESCs (AG-haESCs) can be used for cell-based reverse and forward genetic screens on a whole-genome scale3,4. AG-haESCs, after intracytoplasmic injection into oocytes (referred to as ICAHCI), can be used as a sperm replacement to produce healthy semi-cloned (SC) mice at a rate of ~2% of transferred embryos5,6.

10.1038/cr.2015.132

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