Volume 26, No 2, Feb 2016

ISSN: 1001-0602 
EISSN: 1748-7838 2018 
impact factor 17.848* 
(Clarivate Analytics, 2019)

Volume 26 Issue 2, February 2016: 239-253

ORIGINAL ARTICLES

NudC regulates actin dynamics and ciliogenesis by stabilizing cofilin 1

Cheng Zhang^1,*, Wen Zhang^1,*, Yi Lu^1,*, Xiaoyi Yan^1,2, Xiumin Yan³, Xueliang Zhu³, Wei Liu¹, Yuehong Yang^1,2 and Tianhua Zhou^1,2

¹Department of Cell Biology and Program in Molecular Cell Biology, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310058, China
²Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou, Zhejiang 310003, China
³State Key Laboratory of Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue Yang Road, Shanghai 200031, China Correspondence: Tianhua Zhou, Tel: +86 571 88208258 E-mail: tzhou@zju.edu.cn; Yuehong Yang, Tel: +86 571 88208257(yhyang@zju.edu.cn)

Emerging data indicate that actin dynamics is associated with ciliogenesis. However, the underlying mechanism remains unclear. Here we find that nuclear distribution gene C (NudC), an Hsp90 co-chaperone, is required for actin organization and dynamics. Depletion of NudC promotes cilia elongation and increases the percentage of ciliated cells. Further results show that NudC binds to and stabilizes cofilin 1, a key regulator of actin dynamics. Knockdown of cofilin 1 also facilitates ciliogenesis. Moreover, depletion of either NudC or cofilin 1 causes similar ciliary defects in zebrafish, including curved body, pericardial edema and defective left-right asymmetry. Ectopic expression of cofilin 1 significantly reverses the phenotypes induced by NudC depletion in both cultured cells and zebrafish. Thus, our data suggest that NudC regulates actin cytoskeleton and ciliogenesis by stabilizing cofilin 1.

10.1038/cr.2015.152

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