Volume 27, No 2, Feb 2017
ISSN: 1001-0602
EISSN: 1748-7838 2018
impact factor 17.848*
(Clarivate Analytics, 2019)
Volume 27 Issue 2, February 2017: 298-301 | Open Access
LETTERS TO THE EDITOR
Painting a specific chromosome with CRISPR/Cas9 for live-cell imaging
Yuexin Zhou1,2,3,*, Ping Wang1,2,*, Feng Tian3,4,5,*, Ge Gao3,5, Lei Huang1,2, Wensheng Wei1,2,3,4 and X Sunney Xie1,2,6
1Biodynamics Optical Imaging Center (BIOPIC), School of Life Sciences, Peking University, Beijing 100871, China
2Beijing Advanced Innovation Center for Genomics (ICG), Peking University, Beijing 100871, China
3State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing 100871, China
4Peking-Tsinghua Center for Life Sciences (CLS), Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China
5Center for Bioinformatics, School of Life Sciences, Peking University, Beijing 100871, China
6Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 01238, USA
Correspondence: Lei Huang, E-mail: leihuangmed@gmail.com; Wensheng Wei, E-mail: wswei@pku.edu.cn; X Sunney Xie,(xie@chemistry.harvard.edu)
Visualization of chromosome shapes and dynamics in a live cell is highly desirable and necessary in many areas of cell biology. For example, the copy number of a particular chromosome in cancer cells is often abnormal (e.g., more than two), and therefore probing chromosome copy numbers can aid cancer diagnosis. During interphase, each chromosome exists in its own territory in the nucleus, which can be imaged by fluorescence in situ hybridization (FISH) using sequence-specific probes of different colors1,2. However, such chromosome painting has only been possible in fixed cells, and is not suitable for dynamic monitoring of live cells. Therefore, it would be valuable to visualize DNA replication of one chromosome during interphase, and follow chromosome dynamics in the M phase.
10.1038/cr.2017.9
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