Volume 27, No 9, Sep 2017

ISSN: 1001-0602 
EISSN: 1748-7838 2018 
impact factor 17.848* 
(Clarivate Analytics, 2019)

Volume 27 Issue 9, September 2017: 1128-1141   |  Open Access

ORIGINAL ARTICLES

IPA1 functions as a downstream transcription factor repressed by D53 in strigolactone signaling in rice

Xiaoguang Song¹, Zefu Lu^1,5, Hong Yu¹, Gaoneng Shao^1,2, Jinsong Xiong^1,6, Xiangbing Meng¹, Yanhui Jing¹, Guifu Liu¹, Guosheng Xiong^1,7, Jingbo Duan¹, Xue-Feng Yao³, Chun-Ming Liu³, Hongqing Li⁴, Yonghong Wang¹ and Jiayang Li^1,2

¹State Key Laboratory of Plant Genomics and National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
²University of Chinese Academy of Sciences, Beijing 100049, China
³Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China
⁴Guangdong Provincial Key Lab of Biotechnology for Plant Development, South China Normal University, Guangzhou, Guangdong 510631, China
⁵Present address: Department of Genetics, University of Georgia, Athens, Georgia 30605, USA
⁶Present address: College of Horticulture, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China
⁷Present address: Agricultural Genome Institute at Shenzhen, China Academy of Agricultural Science, Shenzhen, Guangdong 518120, China Correspondence: Jiayang Li, Tel: +86 10 64806577, Fax: +86 10 64806595(jyli@genetics.ac.cn)

Strigolactones (SLs), a group of carotenoid derived terpenoid lactones, are root-to-shoot phytohormones suppressing shoot branching by inhibiting the outgrowth of axillary buds. DWARF 53 (D53), the key repressor of the SL signaling pathway, is speculated to regulate the downstream transcriptional network of the SL response. However, no downstream transcription factor targeted by D53 has yet been reported. Here we report that Ideal Plant Architecture 1 (IPA1), a key regulator of the plant architecture in rice, functions as a direct downstream component of D53 in regulating tiller number and SL-induced gene expression. We showed that D53 interacts with IPA1 in vivo and in vitro and suppresses the transcriptional activation activity of IPA1. We further showed that IPA1 could directly bind to the D53 promoter and plays a critical role in the feedback regulation of SL-induced D53 expression. These findings reveal that IPA1 is likely one of the long-speculated transcription factors that act with D53 to mediate the SL-regulated tiller development in rice.

10.1038/cr.2017.102

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