Volume 28, No 2, Feb 2018
ISSN: 1001-0602
EISSN: 1748-7838 2018
impact factor 17.848*
(Clarivate Analytics, 2019)
Volume 28 Issue 2, February 2018: 249-252
LETTERS TO THE EDITOR
Endothelial-specific m6A modulates mouse hematopoietic stem and progenitor cell development via Notch signaling
Junhua Lv1,4,*, Yifan Zhang1,4,*, Suwei Gao1,5,*, Chunxia Zhang1,4,*, Yusheng Chen3,4,*, Wei Li2,4, Yun-Gui Yang3,4, Qi Zhou2,4, Feng Liu1,4
1 State Key Laboratory of Membrane Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China;
2 State Key Laboratory of Stem Cell and Reproduction Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China;
3 CAS Key Laboratory of Genomic and Precision Medicine, Collaborative Innovation Center of Genetics and Development, College of Future Technology, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China;
4 University of Chinese Academy of Sciences, Beijing 100049, China;
5 College of Life Sciences, Hebei University, Baoding 071002, China
Correspondence: Feng Liu Tel: +86 1064807307; Fax: +86 1064807313(liuf@ioz.ac.cn)
Hematopoietic stem and progenitor cells (HSPCs) have been documented to be specified from hemogenic endothelial (HE) cells in the ventral wall of the dorsal aorta (DA) through the endothelial-to-hematopoietic transition (EHT) during mouse embryogenesis [1]. N6 -methyl-adenosine (m6 A) is the most prevalent mRNA modification in eukaryotes. Although the function of m6 A modification in cell fate determination of embryonic stem cells has been recently reported [2], the physiological role and the underlying mechanism of m6 A modification in definitive hematopoiesis during mouse embryogenesis have not been reported yet. Due to the early lethality of mutant mice with conventional knockout of methyltransferase like 3 (Mettl3) [2], one of the most important m6 A methyltransferase catalytic subunits identified so far [3], here we utilized Vec-Cre mice crossed with Mettl3fl/fl mice to deplete the expression of Mettl3 specifically in endothelial cells of mouse aorta-gonad-mesonephros (AGM) region
10.1038/cr.2017.143
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