Volume 28, No 3, Mar 2018
ISSN: 1001-0602
EISSN: 1748-7838 2018
impact factor 17.848*
(Clarivate Analytics, 2019)
Volume 28 Issue 3, March 2018: 374-378
LETTERS TO THE EDITOR
Molecular basis for CENP-N recognition of CENP-A nucleosome on the human kinetochore
Tian Tian1, 2, *, Xiaorun Li1, 3, *, Yingying Liu1, 4, *, Chengliang Wang1, 2, Xing Liu1, 4, Guoqiang Bi1, 2, Xuan Zhang1, 2, Xuebiao Yao1, 2, 4, Z Hong Zhou1, 3, Jianye Zang1, 2
1Hefei National Laboratory for Physical Sciences at Microscale, CAS Center for Excellence in Biomacromolecules, Collaborative Innovation Center of Chemistry for Life Sciences, and School of Life Sciences, University of Science and Technology of China, 96 Jinzhai Road, Hefei, Anhui 230026, China;
2 Key Laboratory of Structural Biology, Chinese Academy of Sciences, Hefei, Anhui 230027, China;
3 Department of Microbiology, Immunology & Molecular Genetics, University of California, Los Angeles, Los Angeles, CA, USA;
4 Anhui Key Laboratory for Cellular Dynamics & Chemical Biology, Hefei, Anhui 230027, China
Correspondence: Jianye Zang,E-mail: zangjy@ustc.edu.cn; Z Hong Zhou, E-mail: hong.zhou@ucla.edu; Xuebiao Yao,E-mail: yaoxb@ustc.edu.cn; Xuan Zhang,(E-mail: xuanzbin@ustc.edu.cn)
Dear Editor,
Recognition of CENP-A-containing chromatin by CENP-N is a critical step in the assembly of functional kinetochore at the centromere to enable accurate chromosome segregation during cell division [1, 2]. CENP-N is recruited to centromeres during S phase and gradually dissociates during G2 phase. This dynamic assembly of CENP-N onto the centromere is regulated by the change in accessibility to CENP-A’s RG loop (Arg80/Gly81) during the compacting-opening transition of centromeric chromatin structure through the cell cycle[3, 4]. CENP-N has an N-terminal domain that binds to RG loop of CENP-A and a C-terminal domain that interacts with CENP-L to form CENP-N/CENP-L complex (CENP-LN), which further associates with CENP-C and CENP-HIKM to orchestrate kinetochore assembly [1, 3, 5, 6]. Recent studies from hydrogen/deuterium exchange coupled to mass spectrometry (HXMS) have shown that nucleosomal DNA (–21 and –22 nt) had direct contacts with CENP-N in addition to CENP-A’s RG loop [7], suggesting a potential role of CENP-N-DNA interaction in CENP-A nucleosome recognition.
10.1038/cr.2018.13
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