Volume 28, No 7, Jul 2018
ISSN: 1001-0602
EISSN: 1748-7838 2018
impact factor 17.848*
(Clarivate Analytics, 2019)
Volume 28 Issue 7, July 2018: 730-745 | Open Access
ORIGINAL ARTICLES
Spatial transcriptomic survey of human embryonic cerebral cortex by single-cell RNA-seq analysis
Xiaoying Fan 1,2, Ji Dong 1,2, Suijuan Zhong 3,4,5, Yuan Wei 1,6, Qian Wu 3,4,5, Liying Yan 1,6, Jun Yong 1,6, Le Sun 3,4,5, Xiaoye Wang 1,6,Yangyu Zhao 1,6, Wei Wang 1,6, Jie Yan 1,6, Xiaoqun Wang 3,4,5,7, Jie Qiao 1,6,8,9 and Fuchou Tang 1,2,9
1Beijing Advanced Innovation Center for Genomics, Department of Obstetrics and Gynecology, College of Life Sciences, Third Hospital, Peking University, Beijing 100871, China; 2Biomedical Institute for Pioneering Investigation via Convergence and Center for Reproductive Medicine, Ministry of Education Key Laboratory of Cell Proliferation and
Differentiation, Beijing 100871, China; 3State Key Laboratory of Brain and Cognitive Science, CAS Center for Excellence in Brain Science and Intelligence Technology; Institute of
Brain-Intelligence Science and Technology Zhangjiang Laboratory (Shanghai), Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China; 4University of Chinese
Academy of Sciences, Beijing 100049, China; 5Shanghai Center for Brain Science and Intelligence Technology, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China; 6Key Laboratory of Assisted Reproduction, Ministry of Education, Beijing 100191, China; 7Beijing Institute for Brain Disorder, Beijing 100069, China; 8Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology, Beijing 100191, China and 9Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871, China
Correspondence: Correspondence: Xiaoqun Wang (xiaoqunwang@ibp.ac.cn) or Jie Qiao (jie.qiao@263.net) or Fuchou Tang (tangfuchou@pku.edu.cn)These authors contributed equally: Xiaoying Fan, Ji Dong, Suijuan Zhong, and Yuan Wei
The cellular complexity of human brain development has been intensively investigated, although a regional characterization of the entire human cerebral cortex based on single-cell transcriptome analysis has not been reported. Here, we performed RNA-seq on over 4,000 individual cells from 22 brain regions of human mid-gestation embryos. We identified 29 cell sub-clusters, which showed different proportions in each region and the pons showed especially high percentage of astrocytes. Embryonic neurons were not as diverse as adult neurons, although they possessed important features of their destinies in adults. Neuron development was unsynchronized in the cerebral cortex, as dorsal regions appeared to be more mature than ventral regions at this stage. Region-specific genes were comprehensively identified in each neuronal sub-cluster, and a large proportion of these genes were neural disease related. Our results present a systematic landscape of the regionalized gene expression and neuron maturation of the human cerebral cortex.
https://doi.org/10.1038/s41422-018-0053-3
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