Volume 29, No 5, May 2019

ISSN: 1001-0602 
EISSN: 1748-7838 2018 
impact factor 17.848* 
(Clarivate Analytics, 2019)

Volume 29 Issue 5, May 2019: 345-346

RESEARCH HIGHLIGHTS

CasX: a new and small CRISPR gene-editing protein

Hui Yang ¹ and Dinshaw J. Patel ^2,3

¹ State Key Laboratory of Molecular Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai 200031, China; ²Structural Biology Program, Memorial Sloan-Kettering Cancer Center, New York, NY 10065, USA and ³Biology Department, Southern University of Science and Technology, Shenzhen, Guangdong 518055, China
Correspondence: Hui Yang (yanghui@sibcb.ac.cn) or Dinshaw J. Patel (pateld@mskcc.org)

In a recent paper published in Nature , Liu et al. demonstrate the DNA cleavage characteristics of CasX (a newly identified dual-RNA-guided DNA targeting nuclease) and establish its capacity for genome editing in Escherichia coli and human cells. Furthermore, they capture structural snapshots of CasX in different states by cryo-EM and establish a model of RNA assembly, DNA targeting and sequential cleavage.

https://doi.org/10.1038/s41422-019-0165-4

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