Volume 29, No 9, Sep 2019

ISSN: 1001-0602 
EISSN: 1748-7838 2018 
impact factor 17.848* 
(Clarivate Analytics, 2019)

Volume 29 Issue 9, September 2019: 739-753

ORIGINAL ARTICLES

A cyclic nucleotide-gated channel mediates cytoplasmic calcium elevation and disease resistance in rice

Jiachang Wang ^1,2, Xi Liu¹, An Zhang³, Yulong Ren², Fuqing Wu², Gang Wang³, Yang Xu¹, Cailin Lei², Shanshan Zhu², Tian Pan¹, Yongfei Wang¹, Huan Zhang¹, Fan Wang¹, Yan-Qiu Tan³, Yupeng Wang², Xin Jin², Sheng Luo², Chunlei Zhou¹, Xiao Zhang¹, Jinling Liu²,Shuai Wang²
, Lingzhi Meng², Yihua Wang¹, Xi Chen³, Qibing Lin², Xin Zhang², Xiuping Guo², Zhijun Cheng², Jiulin Wang², Yunlu Tian¹, Shijia Liu¹, Ling Jiang¹, Chuanyin Wu², Ertao Wang ³, Jian-Min Zhou ⁴, Yong-Fei Wang ³, Haiyang Wang ² and Jianmin Wan ²

¹National Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing 210095,China; ²National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China; ³National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai 200032, China and ⁴The State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
Correspondence: Jianmin Wan (wanjm@njau.edu.cn)These authors contributed equally: Jiachang Wang, Xi Liu, An Zhang

The transient elevation of cytoplasmic calcium is essential for pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI). However, the calcium channels responsible for this process have remained unknown. Here, we show that rice CDS1 (CELL DEATH and SUSCEPTIBLE to BLAST 1) encoding OsCNGC9, a cyclic nucleotide-gated channel protein, positively regulates the resistance to rice blast disease. We show that OsCNGC9 mediates PAMP-induced Ca2+ influx and that this event is critical for PAMPs-triggered ROS burst and induction of PTI-related defense gene expression. We further show that a PTI-related receptor-like cytoplasmic kinase OsRLCK185 physically interacts with and phosphorylates OsCNGC9 to activate its channel activity. Our results suggest a signaling cascade linking pattern recognition to calcium channel activation, which is required for initiation of PTI and disease resistance in rice.

https://doi.org/10.1038/s41422-019-0219-7

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