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Volume 29, No 10, Oct 2019

ISSN: 1001-0602 
EISSN: 1748-7838 2018 
impact factor 17.848* 
(Clarivate Analytics, 2019)

Volume 29 Issue 10, October 2019: 820-831

ORIGINAL ARTICLES

A cyclic nucleotide-gated channel mediates cytoplasmic calcium elevation and disease resistance in rice

Jiachang Wang 1,2, Xi Liu1, An Zhang3, Yulong Ren2, Fuqing Wu2, Gang Wang3, Yang Xu1, Cailin Lei2, Shanshan Zhu2, Tian Pan1,Yongfei Wang1, Huan Zhang1, Fan Wang1, Yan-Qiu Tan3, Yupeng Wang2, Xin Jin2, Sheng Luo2, Chunlei Zhou1, Xiao Zhang1, Jinling Liu2,Shuai Wang2, Lingzhi Meng2, Yihua Wang1, Xi Chen3, Qibing Lin2, Xin Zhang2, Xiuping Guo2, Zhijun Cheng2, Jiulin Wang2, Yunlu Tian1,Shijia Liu1, Ling Jiang1, Chuanyin Wu2, Ertao Wang 3, Jian-Min Zhou 4, Yong-Fei Wang 3, Haiyang Wang 2 and Jianmin Wan 2

1National Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing 210095,China; 2National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 3National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai 200032, China and 4The State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
 Correspondence: Jianmin Wan (wanjm@njau.edu.cn)These authors contributed equally: Jiachang Wang, Xi Liu, An Zhang

The transient elevation of cytoplasmic calcium is essential for pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI). However, the calcium channels responsible for this process have remained unknown. Here, we show that rice CDS1 (CELL DEATH and SUSCEPTIBLE to BLAST 1) encoding OsCNGC9, a cyclic nucleotide-gated channel protein, positively regulates the resistance to rice blast disease. We show that OsCNGC9 mediates PAMP-induced Ca2+ influx and that this event is critical for PAMPs-triggered ROS burst and induction of PTI-related defense gene expression. We further show that a PTI-related receptor-like cytoplasmic kinase OsRLCK185 physically interacts with and phosphorylates OsCNGC9 to activate its channel activity. Our results suggest a signaling cascade linking pattern recognition to calcium channel activation, which is required for initiation of PTI and disease resistance in rice.


https://doi.org/10.1038/s41422-019-0219-7

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