Volume 29, No 10, Oct 2019
ISSN: 1001-0602
EISSN: 1748-7838 2018
impact factor 17.848*
(Clarivate Analytics, 2019)
Volume 29 Issue 10, October 2019: 870-872
LETTERS TO THE EDITOR
BoneClear: whole-tissue immunolabeling of the intact mouse bones for 3D imaging of neural anatomy and pathology
Qi Wang 1,2,3, Kaili Liu2, Lu Yang 1,4, Huanhuan Wang 1,4 and Jing Yang 1,2,4,5
1State Key Laboratory of Membrane Biology, Peking University,Beijing 100871, China; 2Center for Life Sciences, Peking University,Beijing 100871, China; 3Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China; 4School of Life Sciences, Peking University, Beijing 100871, China and 5IDG/McGovern Institute for Brain Research, Peking University, Beijing 100871, China
These authors contributed equally: Qi Wang, Kaili Liu, Lu Yang,
Huanhuan Wang
Correspondence: Jing Yang (jing.yang@pku.edu.cn)
Dear Editor,
Current approaches to analyze cellular structures in the bone tissues have depended mainly on tissue sections. For instance, a recent study visualized different cellular populations in the 300-μm sections of the mouse femurs1. Similarly, the distribution of hematopoietic stem cells could be examined in the half-bone sections2. Notably, such conventional methods obscure specific cellular structures, e.g., neural innervations, that would be better observed with the intact, unsectioned bones. Moreover, histological sectioning could be challenging for many bone tissues, e.g., vertebral column, skull and limbs.
https://doi.org/10.1038/s41422-019-0217-9
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