Volume 30, No 3, Mar 2020

ISSN: 1001-0602 
EISSN: 1748-7838 2018 
impact factor 17.848* 
(Clarivate Analytics, 2019)

Volume 30 Issue 3, March 2020: 193-194

RESEARCH HIGHLIGHTS

First structural insights into CRISPR-Cas-guided DNA transposition

John van der Oost ¹ and Ioannis Mougiakos ¹

¹ Laboratory of Microbiology, Wageningen University and Research, Stippeneng 4, 6708 WE Wageningen, the Netherlands
Correspondence: John van der Oost (john.vanderoost@wur.nl) or Ioannis Mougiakos (ioannis.mougiakos@wur.nl)

The transposition mechanism of the Vibrio cholerae Tn6677 transposon (VcTn6677) is based on the unique synergy between a classical transposition machinery (TnsABC-TniQ complex) and a nuclease-deficient type I-F CRISPR-associated complex for antiviral defense (VcCascade). Four independent studies, three of which appeared in Cell Research, recently reported structures of the VcCascade, VcCascade-TniQ, and/or VcCascade-TniQ-dsDNA complexes as well as a TniQ dimer complex, providing the first insights into the assembly and DNA targeting mechanism of a CRISPR-associated transposase complex.

https://doi.org/10.1038/s41422-020-0284-y

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