Volume 30, No 3, Mar 2020
ISSN: 1001-0602
EISSN: 1748-7838 2018
impact factor 17.848*
(Clarivate Analytics, 2019)
Volume 30 Issue 3, March 2020: 244-255
ORIGINAL ARTICLES
In vitro testicular organogenesis from human fetal gonads produces fertilization-competent spermatids
Yan Yuan 1,2, Laihua Li2, Qing Cheng3, Feiyang Diao4, Qiao Zeng4, Xiaoyu Yang4, Yibo Wu1, Hao Zhang 2, Mingqian Huang2,Junqing Chen2, Quan Zhou2, Yunfei Zhu2, Rong Hua2, Jianyu Tian2, Xin Wang2, Zuomin Zhou2, Jie Hao5, Jinjin Yu1, Dong Hua1,Jiayin Liu4, Xuejiang Guo 2, Qi Zhou 5 and Jiahao Sha 2,3
1 Human Reproductive and Genetic Center, Affiliated Hospital of Jiangnan University, 214062 Wuxi, Jiangsu, China; 2State Key Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University, 211166 Nanjing, Jiangsu, China; 3State Key Laboratory of Reproductive Medicine, Women’s Hospital of Nanjing Medical University, Nanjing Medical University, 210029 Nanjing, Jiangsu, China; 4State Key Laboratory of Reproductive Medicine, Clinical Center of Reproductive Medicine, First Affiliated Hospital of Nanjing Medical University, Nanjing Medical University, 210029 Nanjing, Jiangsu, China and 5State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, 100101 Beijing, China
Correspondence: Jiahao Sha (shajh@njmu.edu.cn) or Qi Zhou (zhouqi@ioz.ac.cn) or Xuejiang Guo (guo_xuejiang@njmu.edu.cn)These authors contributed equally: Yan Yuan, Laihua Li, Qing Cheng, Feiyang Diao, Qiao Zeng
Unlike most organs that mature during the fetal period, the male reproductive system reaches maturity only at puberty with the commencement of spermatogenesis. Robust modelling of human testicular organogenesis in vitro would facilitate research into mechanisms of and factors affecting human spermatogenic failure and male fertility preservation in prepubertal tumor patients. Here, we report successful recapitulation of human testicular organogenesis in vitro from fetal gonadal ridge. Our model displayed the formation of mature seminiferous epithelium and self-renewing spermatogonia. Remarkably, in vitro-derived haploid spermatids have undergone meiotic recombination, and showed increased genetic diversity as indicated by genetic analysis. Moreover, these spermatids were able to fertilize oocytes and support subsequent blastocyst formation. The in vitro testicular organogenesis system described here will play an important role in elucidating the regulation of human testis development and maintaining male fertility in prepubertal cancer patients.
https://doi.org/10.1038/s41422-020-0283-z
FULL TEXT | PDF
Browse 960
