Volume 30, No 4, Apr 2020

ISSN: 1001-0602 
EISSN: 1748-7838 2018 
impact factor 17.848* 
(Clarivate Analytics, 2019)

Volume 30 Issue 4, April 2020: 356-359   |  Open Access

LETTERS TO THE EDITOR

In vitro expansion of human sperm through nuclear transfer

Xiaoyu Merlin Zhang¹, Keliang Wu², Yuxuan Zheng ^3,4,5, Han Zhao², Junpeng Gao ^3,4, Zhenzhen Hou², Meiling Zhang⁶,Jiaoyang Liao¹, Jingye Zhang², Yuan Gao², Yuanyuan Li¹, Lin Li³,Fuchou Tang ^3,4,5, Zi-Jiang Chen² and Jinsong Li ^1,7

¹State Key Laboratory of Cell Biology, Shanghai Key Laboratory of Molecular Andrology, Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, 320 Yueyang Road, Shanghai 200031, China; ²Center for Reproductive Medicine, Shandong University, Jinan, Shandong 250001, China; ³Beijing Advanced Innovation Center for Genomics, College of Life Sciences, Peking University, Beijing 100871, China; ⁴Biomedical
Institute for Pioneering Investigation via Convergence, Ministry of
Education Key Laboratory of Cell Proliferation and Differentiation,
Beijing 100871, China; ⁵Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China; ⁶Center for Reproductive Medicine, Shanghai Key Laboratory for Assisted Reproduction and Reproductive Genetics, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China and ⁷ School of Life Science and Technology, Shanghai Tech University, 100 Haike Road, Shanghai 201210,, China.
These authors contributed equally: Xiaoyu Merlin Zhang, Keliang Wu,
Yuxuan Zheng, Han Zhao
Correspondence: Fuchou Tang (tangfuchou@pku.edu.cn) orZi-Jiang Chen (chenzijiang@hotmail.com) or Jinsong Li (jsli@sibcb.ac.cn)

Dear Editor,

Mammalian haploid embryonic stem cells (haESCs) represent an ideal tool for genetic analysis due to the presence of only one set of genetic materials. HaESCs fall into two readily distinguishable groups based on their genome origins: parthenogenetic haESCs (PG-haESCs) that are derived from oocyte-originated parthenogenetic embryos and androgenetic haESCs (AG-haESCs) that are produced through sperm nuclear transfer. Both PG- and AG-haESCs are feasible for delineating genome function at cellular level in vitro. Importantly, AG-haESCs can be used as a sperm replacement and applied to deciphering gene function at organismal level when combined with CRISPR-Cas9 technology.1 However, while PG-haESCs have been generated from both rodent and primates,2,3,4,5 AG-haESCs can only be obtained in rodent to date.6,7

1I1Cell Research, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 319 Yueyang Road, Shanghai 200031,China

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