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Volume 30, No 6, Jun 2020

ISSN: 1001-0602 
EISSN: 1748-7838 2018 
impact factor 17.848* 
(Clarivate Analytics, 2019)

Volume 30 Issue 6, June 2020: 544-547

LETTERS TO THE EDITOR

METTL4 is an snRNA m6Am methyltransferase that regulates RNA splicing

Hao Chen 1, Lei Gu1, Esteban A. Orellana 2, Yuanyuan Wang3,Jiaojiao Guo4, Qi Liu2, Longfei Wang 5,6 , Zhangfei Shen 1,5,6 ,Hao Wu 5,6 , Richard I. Gregory2, Yi Xing 7,8 and Yang Shi 1

1Division of Newborn Medicine and Program in Epigenetics, Boston Children’s Hospital, Harvard Medical School, 300 Longwood Avenue, Boston, MA 02115, USA; 2Stem Cell Program, Division of Hematology/Oncology, Boston Children’s Hospital, Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School,Boston, MA 02115, USA; 3Bioinformatics Interdepartmental Graduate Program, University of California, Los Angeles, Los Angeles, CA 90095, USA; 4College of Science, Northeastern University, 360 Huntington Ave., Boston, MA 02115, USA; 5Program in Cellular and Molecular Medicine, Boston Children’s Hospital, Boston, MA 02115, USA; 6Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA; 7Center for Computational and Genomic Medicine, The Children’s Hospital of Philadelphia, Philadelphia, PA 19104, USA and 8Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
These authors contributed equally: Hao Chen, Lei Gu
Correspondence: Yang Shi (yshi@hms.harvard.edu)

Dear Editor,

Protein coding mRNAs and non-coding RNAs are extensively modified by chemical modifications,1 including methylation of the N6 position of adenosine (m6A).2 While mRNA internal m6A methylation is mediated by METTL3 and METTL14, which are members of the MT-A70 family,2 N6-methylation of cap-adjacent N6,2′-O-dimethyladenosine (m6Am) is catalyzed by PCIF1.3,4,5,6 Non-coding RNAs are also m6Am methylated internally,7 but the corresponding enzyme remains unknown. Here we provide evidence that METTL4 mediates internal m6Am methylation of U2 small nuclear RNA (snRNA).


Cell Research (2006) 16:799-808. doi:10.1038/sj.cr.7310097; published online 3 October 2006

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