Volume 30, No 5, May 2020
ISSN: 1001-0602
EISSN: 1748-7838 2018
impact factor 17.848*
(Clarivate Analytics, 2019)
Volume 30 Issue 5, May 2020: 421-435 | Open Access
ORIGINAL ARTICLES
The PIWI protein Aubergine recruits eIF3 to activate translation in the germ plasm
Anne Ramat1, Maria-Rosa Garcia-Silva1, Camille Jahan1, Rima Naït-Saïdi1, Jérémy Dufourt 1,5 , Céline Garret1, Aymeric Chartier1, Julie Cremaschi1, Vipul Patel1, Mathilde Decourcelle 2, Amandine Bastide3, François Juge 4 and Martine Simonelig 1
1 mRNA Regulation and Development, Institute of Human Genetics, UMR9002 CNRS-Univ Montpellier, 141 rue de la Cardonille, 34396 Montpellier Cedex 5, France; 2BioCampus Montpellier, CNRS, INSERM, Univ Montpellier, Montpellier, France; 3IGF, Univ Montpellier, CNRS, INSERM, Montpellier, France and 4Institut de Génétique Moléculaire de Montpellier, Univ Montpellier, CNRS, Montpellier, France
Correspondence: Martine Simonelig (Martine.Simonelig@igh.cnrs.fr)5 Present address: Institut de Génétique Moléculaire de Montpellier, Univ Montpellier, CNRS, Montpellier, FranceThese authors contributed equally: Anne Ramat, Maria-Rosa Garcia-Silva
Piwi-interacting RNAs (piRNAs) and PIWI proteins are essential in germ cells to repress transposons and regulate mRNAs. In Drosophila, piRNAs bound to the PIWI protein Aubergine (Aub) are transferred maternally to the embryo and regulate maternal mRNA stability through two opposite roles. They target mRNAs by incomplete base pairing, leading to their destabilization in the soma and stabilization in the germ plasm. Here, we report a function of Aub in translation. Aub is required for translational activation of nanos mRNA, a key determinant of the germ plasm. Aub physically interacts with the poly(A)-binding protein (PABP) and the translation initiation factor eIF3. Polysome gradient profiling reveals the role of Aub at the initiation step of translation. In the germ plasm, PABP and eIF3d assemble in foci that surround Aub-containing germ granules, and Aub acts with eIF3d to promote nanos translation. These results identify translational activation as a new mode of mRNA regulation by Aub, highlighting the versatility of PIWI proteins in mRNA regulation.
Cell Research advance online publication 6 Jun 2006; doi: 10.1038/sj.cr.7310067
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