Volume 30, No 5, May 2020

ISSN: 1001-0602 
EISSN: 1748-7838 2018 
impact factor 17.848* 
(Clarivate Analytics, 2019)

Volume 30 Issue 5, May 2020: 452-454   |  Open Access

LETTERS TO THE EDITOR

Structural basis for gating mechanism of Pannexin 1 channel

Luqiu Mou¹ , Meng Ke^2,3,4 , Mengxiao Song^1,5 , Yuanyue Shan^2,3,4 , Qingjie Xiao¹ , Qingting Liu¹ , Jialu Li¹ , Ke Sun^1,5 , Lei Pu^1,5 , Li Guo¹ , Jia Geng^1,5,†,* , Jianping Wu^2,3,4,†,* , Dong Deng^1,†,*

¹1. Division of Obstetrics, Key Laboratory of Birth Defects and Related Disease of Women and Children of MOE, State Key Laboratory of Biotherapy, West China Second Hospital, Sichuan University, Chengdu, 610041 Sichuan, China;
²School of Life Sciences, Westlake University, Hangzhou, 310024 Zhejiang, China;
³Institute of Biology, Westlake Institute for Advanced Study, Hangzhou, 310024 Zhejiang, China;
⁴Key Laboratory of Structural Biology of Zhejiang Province, School of Life Sciences, Westlake University, Hangzhou, 310024 Zhejiang, China;
⁵Department of Laboratory Medicine, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University and Collaborative Innovation Center for Biotherapy, Chengdu, 610041 Sichuan, China
^†These authors contributed equally
Correspondence: Jia Geng(geng.jia@scu.edu.cn)Jianping Wu(wujianping@westlake.edu.cn)Dong Deng(dengd@scu.edu.cn)

Dear Editor,

Pannexin 1 (PANX1) plays extensive physiological roles across diverse fields of biology, including cell death,1,2 inflammation,3 cancer progression,4 and neurological disorders.5 As suggested by biochemical analysis, PANX1 forms an oligomeric channel for the facilitated diffusion of ions and large molecules across the plasma membrane upon activation.6 However, the underlying molecular gating mechanism of PANX1 and the structure of human PANX1 are still elusive. In the current study, we report the cryo-EM structures of full-length and carboxyl-terminal (CT) tail-cleaved human PANX1. Combined with single-channel electrophysiological study, we identified the key residues involved in the gating of PANX1.

https://doi.org/10.1038/s41422-020-0313-x

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