Volume 31, No 7, Jul 2021

ISSN: 1001-0602 
EISSN: 1748-7838 2018 
impact factor 17.848* 
(Clarivate Analytics, 2019)

Volume 31 Issue 7, July 2021: 821-824

LETTERS TO THE EDITOR

Solution structure of the voltage-gated Tim23 channel in complex with a mitochondrial presequence peptide

Shu Zhou^1,2,* , Maosen Ruan^1,2 , Yunyan Li¹ , Jing Yang¹ , Suwen Bai³ , Christian Richter⁴ , Harald Schwalbe⁴ , Can Xie^1,2 , Bing Shen^3,* , Junfeng Wang^1,2,5,*

¹High Magnetic Field Laboratory, CAS Key Laboratory of High Magnetic Field and Ion Beam Physical Biology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, Anhui 230031, China
²University of Science and Technology of China, Hefei, Anhui 230026, China
³School of Basic Medical Sciences, Anhui Medical University, Hefei, Anhui 230032, China
⁴Center for Biomolecular Magnetic Resonance, Institute for Organic Chemistry and Chemical Biology, Goethe-University Frankfurt, 60438 Frankfurt, Germany
⁵Institutes of Physical Science and Information Technology, Anhui University, Hefei, Anhui 230601, China
These authors contributed equally: Shu Zhou, Maosen Ruan Correspondence: Shu Zhou(shu.zhou@hmfl.ac.cn)Bing Shen(shenbing@ahmu.edu.cn)Junfeng Wang(junfeng@hmfl.ac.cn)

Dear Editor,

Most mitochondrial proteins are synthesized in the cytosol and transported into various mitochondrial subcompartments in a process that is mediated by intricate multimeric machineries. Tim23, the key component of the TIM23 complex forming a channel in the mitochondrial inner membrane (MIM), is believed to recognize and translocate precursor proteins into the mitochondrial matrix or to release them into the MIM.1 Previous study has demonstrated that purified Tim23 forms a protein-conducting channel that can be activated by an appropriate change in the membrane potential in the presence of a mimetic mitochondrial preprotein,2 a signaling peptide corresponding to the presequence of cytochrome c oxidase subunit IV (denoted as pCoxIV, primary sequence: MLSLRQSIRFFKPATRTLCSSRYLL). Here, we present the three-dimensional (3D) solution structure of the yeast Tim23 channel in complex with pCoxIV in micelles as determined by high-resolution nuclear magnetic resonance (NMR), providing structural insight into the molecular mechanism of presequence translocation.

https://doi.org/10.1038/s41422-020-00452-y

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