Volume 32, No 4, Apr 2022

ISSN: 1001-0602 
EISSN: 1748-7838 2018 
impact factor 17.848* 
(Clarivate Analytics, 2019)

Volume 32 Issue 4, April 2022: 329-330

RESEARCH HIGHLIGHTS

Transcriptomic profiling fuels the derivation of stable pig epiblast stem cells

Cuiqing Zhong^1,† , Ronghui Li^2,† , Juan Carlos Izpisua Belmonte^1,*

¹Altos Labs, San Diego, CA, USA
²Salk Institute for Biological Studies, La Jolla, CA, USA
^†These authors contributed equally: Cuiqing Zhong, Ronghui Li
Correspondence: Juan Carlos Izpisua Belmonte(jcbelmonte@altoslabs.com)

It is controversial whether pig pluripotent stem cells can be derived and stably maintained in a culture dish. In a recent paper published in Cell Research , Zhi et al. perform a comprehensive transcriptome landscape analysis of pig embryos at all developmental stages from embryonic (E) day 0 to E14 and identify a culture cocktail to stabilize pig pregastrulation epiblast stem cells (pgEpiSCs) in vitro for long-term passaging; the pgEpiSCs can undergo multiple rounds of gene editing and generate cloned animals.

https://doi.org/10.1038/s41422-021-00609-3

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