Volume 32, No 4, Apr 2022

ISSN: 1001-0602 
EISSN: 1748-7838 2018 
impact factor 17.848* 
(Clarivate Analytics, 2019)

Volume 32 Issue 4, April 2022: 349-358   |  Open Access

ORIGINAL ARTICLES

Cryo-EM structure of the nuclear ring from Xenopus laevis nuclear pore complex

Gaoxingyu Huang^1,2,3,†,* , Xiechao Zhan^1,2,3,† , Chao Zeng^1,2,3,† , Xuechen Zhu^1,2,3,† , Ke Liang^1,2,3,† , Yanyu Zhao^1,2,3 , Pan Wang^4,5 , Qifan Wang^1,2,3 , Qiang Zhou^1,2,3 , Qinghua Tao⁴ , Minhao Liu⁴ , Jianlin Lei⁴ , Chuangye Yan^4,5 , Yigong Shi^1,2,3,4,5,*

¹Westlake Laboratory of Life Sciences and Biomedicine, 18 Shilongshan Road, Hangzhou, Zhejiang, China
²Key Laboratory of Structural Biology of Zhejiang Province, School of Life Sciences, Westlake University, 18 Shilongshan Road, Hangzhou, Zhejiang, China
³Institute of Biology, Westlake Institute for Advanced Study, 18 Shilongshan Road, Hangzhou, Zhejiang, China
⁴Beijing Advanced Innovation Center for Structural Biology & Frontier Research Center for Biological Structure, Tsinghua University, Beijing, China
⁵Tsinghua University-Peking University Joint Center for Life Sciences; School of Life Sciences, Tsinghua University, Beijing, China
^†These authors contributed equally: Gaoxingyu Huang, Xiechao Zhan, Chao Zeng, Xuechen Zhu, Ke Liang
Correspondence: Gaoxingyu Huang(huanggaoxingyu@westlake.edu.cn)Yigong Shi(syg@westlake.edu.cn)

Nuclear pore complex (NPC) shuttles cargo across the nuclear envelope. Here we present single-particle cryo-EM structure of the nuclear ring (NR) subunit from Xenopus laevis NPC at an average resolution of 5.6 Å. The NR subunit comprises two 10-membered Y complexes, each with the nucleoporin ELYS closely associating with Nup160 and Nup37 of the long arm. Unlike the cytoplasmic ring (CR) or inner ring (IR), the NR subunit contains only one molecule each of Nup205 and Nup93. Nup205 binds both arms of the Y complexes and interacts with the stem of inner Y complex from the neighboring subunit. Nup93 connects the stems of inner and outer Y complexes within the same NR subunit, and places its N-terminal extended helix into the axial groove of Nup205 from the neighboring subunit. Together with other structural information, we have generated a composite atomic model of the central ring scaffold that includes the NR, IR, and CR. The IR is connected to the two outer rings mainly through Nup155. This model facilitates functional understanding of vertebrate NPC.

https://doi.org/10.1038/s41422-021-00610-w

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