Volume 32, No 5, May 2022

ISSN: 1001-0602 
EISSN: 1748-7838 2018 
impact factor 17.848* 
(Clarivate Analytics, 2019)

Volume 32 Issue 5, May 2022: 437-447   |  Open Access

ORIGINAL ARTICLES

Near-atomic structure of the inner ring of the Saccharomyces cerevisiae nuclear pore complex

Zongqiang Li^1,2,† , Shuaijiabin Chen^1,2,† , Liang Zhao^1,2,† , Guoqiang Huang^1,2,† , Xiong Pi¹ , Shan Sun¹ , Peiyi Wang^2,3 , Sen-Fang Sui^1,2,3,*

¹State Key Laboratory of Membrane Biology, Beijing Advanced Innovation Center for Structural Biology, Beijing Frontier Research Center for Biological Structure, School of Life Sciences, Tsinghua University, Beijing, China
²Department of Biology, Southern University of Science and Technology, Shenzhen, Guangdong, China
³Cryo-EM Center, Southern University of Science and Technology, Shenzhen, Guangdong, China
^†These authors contributed equally: Zongqiang Li, Shuaijiabin Chen, Liang Zhao and Guoqiang Huang
Correspondence: Sen-Fang Sui(suisf@mail.tsinghua.edu.cn)

Nuclear pore complexes (NPCs) mediate bidirectional nucleocytoplasmic transport of substances in eukaryotic cells. However, the accurate molecular arrangement of NPCs remains enigmatic owing to their huge size and highly dynamic nature. Here we determined the structure of the asymmetric unit of the inner ring (IR monomer) at 3.73 Å resolution by single-particle cryo-electron microscopy, and created an atomic model of the intact IR consisting of 192 molecules of 8 nucleoporins. In each IR monomer, the Z-shaped Nup188–Nup192 complex in the middle layer is sandwiched by two approximately parallel rhomboidal structures in the inner and outer layers, while Nup188, Nup192 and Nic96 link all subunits to constitute a relatively stable IR monomer. In contrast, the intact IR is assembled by loose and instable interactions between IR monomers. These structures, together with previously reported structural information of IR, reveal two distinct interaction modes between IR monomers and e

https://doi.org/10.1038/s41422-022-00632-y

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