Volume 32, No 10, Oct 2022

ISSN: 1001-0602 
EISSN: 1748-7838 2018 
impact factor 17.848* 
(Clarivate Analytics, 2019)

Volume 32 Issue 10, October 2022: 875-877

RESEARCH HIGHLIGHTS

Structure-based evolutionary relationship between IscB and Cas9

Ning Jia^1,* , Dinshaw J. Patel^2,*

¹Department of Biochemistry, School of Medicine, Shenzhen Key Laboratory of Cell Microenvironment, Guangdong Provincial Key Laboratory of Cell Microenvironment and Disease Research, Southern University of Science and Technology, Shenzhen, Guangdong, China
²Structural Biology Program, Memorial Sloan-Kettering Cancer Center, New York, NY, USA
Correspondence: Ning Jia(jian@sustech.edu.cn)Dinshaw J. Patel(pateld@mskcc.org)

In a recent study published in Science, Ailong Ke and coworkers determined the cryo-EM structure of the dsDNAbound IscBωRNA ribonucleoprotein (RNP) complex, which was supplemented with complementary biochemical experiments, revealing the architectural and mechanistic similarities for RNA-guided DNA recognition and activation-mediated cleavage by IscB and Cas9 RNPs. 

https://doi.org/10.1038/s41422-022-00709-8

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