Volume 32, No 12, Dec 2022

ISSN: 1001-0602 
EISSN: 1748-7838 2018 
impact factor 17.848* 
(Clarivate Analytics, 2019)

Volume 32 Issue 12, December 2022: 1044-1046

RESEARCH HIGHLIGHTS

A type III-E CRISPR Craspase exhibiting RNase and protease activities

Hui Yang^1,* , Dinshaw J. Patel^2,*

¹State Key Laboratory of Molecular Biology, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai, China
²Structural Biology Program, Memorial Sloan-Kettering Cancer Center, New York, NY, USA
Correspondence: Hui Yang(yanghui@sibcb.ac.cn)Dinshaw J. Patel(pateld@mskcc.org)

Type III-E CRISPR-Cas effector gRAMP/Cas7-11 is a RNA-guided RNA endonuclease associated with a putative caspase-like protease TRP-CHAT/Csx29, which targets endogenous protein substrate Csx30. In a recent paper published in Science, the Ailong Ke lab has captured cryo-EM snapshots of the nuclease–protease complex Craspase in both resting and different functional states; this paper together with four other related studies have elucidated the molecular mechanisms of both target RNA cleavage and RNA-mediated allosteric regulation of protease catalytic activity, thereby highlighting RNA-dependent protease-based programmed cell death.

https://doi.org/10.1038/s41422-022-00739-2

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