Volume 35, No 4, Apr 2025

ISSN: 1001-0602 
EISSN: 1748-7838 2018 
impact factor 17.848* 
(Clarivate Analytics, 2019)

Volume 35 Issue 4, April 2025: 309-312   |  Open Access

LETTERS TO THE EDITOR

The conjugation-associated linear-BAC iterative assembling (CALBIA) method for cloning 2.1-Mb human chromosomal DNAs in bacteria

Li Zhong^1,† , Qi Zhang^1,† , Ning Lu^1,† , Tao Wang¹ , Xiaoli Xue^2,* , Zhongjun Qin^1,*

¹Key Laboratory of Synthetic Biology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai, China
²State Key Laboratory of Microbial Metabolism, and School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, Shanghai, China
^†These authors contributed equally: Li Zhong, Qi Zhang, Ning Lu
Correspondence: Xiaoli Xue(xlxue@sjtu.edu.cn)Zhongjun Qin(qin@cemps.ac.cn)

https://doi.org/10.1038/s41422-024-01063-7

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